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Molecular Dynamics Inc imagequant 5 2 software
Imagequant 5 2 Software, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imagequant+5+2+software/imagequant+software/pm41913238-66-12-15
Average 86 stars, based on 1 article reviews
imagequant 5 2 software - by Bioz Stars, 2026-10
86/100 stars

Images

Related Articles

Software:

Article Title: FGF21 promotes the resolution of inflammation through the ALOX15/SPM pathway in acute respiratory distress syndrome.
Article Snippet: Fluorescence signals in cells were observed using a Leica TCS SP8 confocal microscope (Leica, Germany) and .. AR TIC LE IN PR ES S ARTICLE IN PRESS quantified using ImageQuant 5.2 software (Molecular Dynamics, USA). ..

Article Title: Illuminating the Roles of Outer Membrane Proteins and Outer Membrane Vesicles in Vibrio Cholerae Biofilm
Article Snippet: .. The immunoblots were developed with the SuperSignal West Pico chemiluminescent kit (Pierce) and 37 quantified using ImageQuant 5.2 software (Molecular Dynamics). ..

Article Title: Rational Design of RNA Editing Guide Strands: Cytidine Analogs at the Orphan Position
Article Snippet: SDS-polyacrylamide gels were visualized with a Molecular Dynamics 9400 Typhon phosphorimager. .. Data were analyzed with Molecular Dynamics ImageQuant 5.2 software. .. All MALDI analyses were performed at the University of California, Davis Mass Spectrometry Facilities using a Bruker UltraFlextreme MALDI TOF/TOF mass spectrometer.

Article Title: Yeast mitochondrial protein Pet111p binds directly to two distinct targets in COX2 mRNA, suggesting a mechanism of translational activation
Article Snippet: .. The radioactive RNA species were visualized by phosphor imaging with a Typhoon 9410 scanner (GE Healthcare) and quantified using ImageQuant 5.2 software (Molecular Dynamics). by guest on M arch 29, 2019 http://w w w .jbc.org/ D ow nloaded from Acknowledgements: PAR-CLIP sequencing data were deposited to the NCBI Gene Expression Omnibus (GSE117899). ..

Article Title: Histone demethylase KDM2A is a selective vulnerability of cancers relying on alternative telomere maintenance
Article Snippet: Oligonucleotide probe for telomere or Alu repeats was labeled with [ P]-ATP (3,000 Ci/mmol) and T4 nucleotide kinase (New England Biolabs). .. The membrane was hybridized in Church hybridization buffer containing a P-labeled probe at 42°C overnight, washed three times in 0.04 N Na-phosphate, 1% SDS, 1 mM EDTA at 42°C, developed with a Typhoon 9410 Imager (GE Healthcare Life Sciences) and quantified with ImageQuant 5.2 software (Molecular Dynamics). .. Antibodies used in ChIP assay were anti-FLAG (F1804, Sigma), anti-H3K36me2 (2901, Cell Signaling), and mouse IgG (sc2025, Santa Cruz Biotechnology).

Article Title: ARID1A promotes genomic stability through protecting telomere cohesion
Article Snippet: .. The images were analyzed by Phosphor-imager, visualized by Typhoon 9410 Imager (GE Healthcare), and processed with ImageQuant 5.2 software (Molecular Dynamics). ..

Article Title: Whole-Exome Sequencing Identifies Homozygote Nonsense Variants in LMOD2 Gene Causing Infantile Dilated Cardiomyopathy.
Article Snippet: Conjugated secondary antibodies (Bio-Rad, Hercules, CA, USA) were used followed by an ECL reaction to develop the blots according to the manufacturer’s instructions. .. Band intensities from the film were analyzed by IMAGEQUANT 5.2 software (Molecular Dynamics) if needed. .. For Lmod2 expression, AP52508PU-N (OriGene, Rockville, MD, USA) was used with Gapdh (Cell signaling, Danvers, MA, USA, catalog no. 2118S) as a loading control.

Western Blot:

Article Title: Illuminating the Roles of Outer Membrane Proteins and Outer Membrane Vesicles in Vibrio Cholerae Biofilm
Article Snippet: .. The immunoblots were developed with the SuperSignal West Pico chemiluminescent kit (Pierce) and 37 quantified using ImageQuant 5.2 software (Molecular Dynamics). ..

Imaging:

Article Title: Yeast mitochondrial protein Pet111p binds directly to two distinct targets in COX2 mRNA, suggesting a mechanism of translational activation
Article Snippet: .. The radioactive RNA species were visualized by phosphor imaging with a Typhoon 9410 scanner (GE Healthcare) and quantified using ImageQuant 5.2 software (Molecular Dynamics). by guest on M arch 29, 2019 http://w w w .jbc.org/ D ow nloaded from Acknowledgements: PAR-CLIP sequencing data were deposited to the NCBI Gene Expression Omnibus (GSE117899). ..

Sequencing:

Article Title: Yeast mitochondrial protein Pet111p binds directly to two distinct targets in COX2 mRNA, suggesting a mechanism of translational activation
Article Snippet: .. The radioactive RNA species were visualized by phosphor imaging with a Typhoon 9410 scanner (GE Healthcare) and quantified using ImageQuant 5.2 software (Molecular Dynamics). by guest on M arch 29, 2019 http://w w w .jbc.org/ D ow nloaded from Acknowledgements: PAR-CLIP sequencing data were deposited to the NCBI Gene Expression Omnibus (GSE117899). ..

Gene Expression:

Article Title: Yeast mitochondrial protein Pet111p binds directly to two distinct targets in COX2 mRNA, suggesting a mechanism of translational activation
Article Snippet: .. The radioactive RNA species were visualized by phosphor imaging with a Typhoon 9410 scanner (GE Healthcare) and quantified using ImageQuant 5.2 software (Molecular Dynamics). by guest on M arch 29, 2019 http://w w w .jbc.org/ D ow nloaded from Acknowledgements: PAR-CLIP sequencing data were deposited to the NCBI Gene Expression Omnibus (GSE117899). ..

Membrane:

Article Title: Histone demethylase KDM2A is a selective vulnerability of cancers relying on alternative telomere maintenance
Article Snippet: Oligonucleotide probe for telomere or Alu repeats was labeled with [ P]-ATP (3,000 Ci/mmol) and T4 nucleotide kinase (New England Biolabs). .. The membrane was hybridized in Church hybridization buffer containing a P-labeled probe at 42°C overnight, washed three times in 0.04 N Na-phosphate, 1% SDS, 1 mM EDTA at 42°C, developed with a Typhoon 9410 Imager (GE Healthcare Life Sciences) and quantified with ImageQuant 5.2 software (Molecular Dynamics). .. Antibodies used in ChIP assay were anti-FLAG (F1804, Sigma), anti-H3K36me2 (2901, Cell Signaling), and mouse IgG (sc2025, Santa Cruz Biotechnology).

Hybridization:

Article Title: Histone demethylase KDM2A is a selective vulnerability of cancers relying on alternative telomere maintenance
Article Snippet: Oligonucleotide probe for telomere or Alu repeats was labeled with [ P]-ATP (3,000 Ci/mmol) and T4 nucleotide kinase (New England Biolabs). .. The membrane was hybridized in Church hybridization buffer containing a P-labeled probe at 42°C overnight, washed three times in 0.04 N Na-phosphate, 1% SDS, 1 mM EDTA at 42°C, developed with a Typhoon 9410 Imager (GE Healthcare Life Sciences) and quantified with ImageQuant 5.2 software (Molecular Dynamics). .. Antibodies used in ChIP assay were anti-FLAG (F1804, Sigma), anti-H3K36me2 (2901, Cell Signaling), and mouse IgG (sc2025, Santa Cruz Biotechnology).

other:

Article Title: Rational Design of Oligonucleotide Guide Strands for Site-Directed RNA Editing
Article Snippet: Unless otherwise stated, all reagents were purchased from commercial sources (Fisher Scientific or Sigma Aldrich) and were used without further purification.



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IL-2 promoter DNA binding of modified NF- κ Bs expressed in a cell-free E. coli protein biosynthesizing system following treatment with a cytoplasmic lysate from activated Jurkat cells in the presence of GTP. The relative intensities were determined using ImageQuant version 5.2 software (Molecular Dynamics) on the basis of the 32 P signal. The value of the band for wild-type NF- κ B in the presence of GTP was defined as 100%. (A) IL-2 promoter DNA binding with NF- κ Bs depends on the presence of GTP and cytoplasmic lysate. Lanes 1–3, wild-type NF- κ B; lanes 4–15, modified NF- κ Bs having p50 subunits with pTyr at respective positions 44, 60, 82, or 90. (B) Comparison of IL-2 promoter DNA binding ability to wild-type and modified NF- κ Bs. Controls included wild-type NF- κ B in the absence and presence of GTP (lanes 1 and 2), and NF κ Bs containing modified p50 subunits with pTyr at positions 44, 60, 82, or 90 (lanes 3, 4, 5, and 6, respectively). The statistical significance was determined using the Student’s t -test: wild-type vs pTyr44-p50, p > 0.05; wild-type vs pTyr60-p50, p < 0.001; wild-type vs pTyr82-p50, p < 0.001; and wild-type vs pTyr90-p50, p < 0.001.
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IL-2 promoter DNA binding of modified NF- κ Bs expressed in a cell-free E. coli protein biosynthesizing system following treatment with a cytoplasmic lysate from activated Jurkat cells in the presence of GTP. The relative intensities were determined using ImageQuant version 5.2 software (Molecular Dynamics) on the basis of the 32 P signal. The value of the band for wild-type NF- κ B in the presence of GTP was defined as 100%. (A) IL-2 promoter DNA binding with NF- κ Bs depends on the presence of GTP and cytoplasmic lysate. Lanes 1–3, wild-type NF- κ B; lanes 4–15, modified NF- κ Bs having p50 subunits with pTyr at respective positions 44, 60, 82, or 90. (B) Comparison of IL-2 promoter DNA binding ability to wild-type and modified NF- κ Bs. Controls included wild-type NF- κ B in the absence and presence of GTP (lanes 1 and 2), and NF κ Bs containing modified p50 subunits with pTyr at positions 44, 60, 82, or 90 (lanes 3, 4, 5, and 6, respectively). The statistical significance was determined using the Student’s t -test: wild-type vs pTyr44-p50, p > 0.05; wild-type vs pTyr60-p50, p < 0.001; wild-type vs pTyr82-p50, p < 0.001; and wild-type vs pTyr90-p50, p < 0.001.

Journal: ACS chemical biology

Article Title: Site-Selective Tyrosine Phosphorylation in the Activation of the p50 Subunit of NF- κ B for DNA Binding and Transcription

doi: 10.1021/acschembio.2c00678

Figure Lengend Snippet: IL-2 promoter DNA binding of modified NF- κ Bs expressed in a cell-free E. coli protein biosynthesizing system following treatment with a cytoplasmic lysate from activated Jurkat cells in the presence of GTP. The relative intensities were determined using ImageQuant version 5.2 software (Molecular Dynamics) on the basis of the 32 P signal. The value of the band for wild-type NF- κ B in the presence of GTP was defined as 100%. (A) IL-2 promoter DNA binding with NF- κ Bs depends on the presence of GTP and cytoplasmic lysate. Lanes 1–3, wild-type NF- κ B; lanes 4–15, modified NF- κ Bs having p50 subunits with pTyr at respective positions 44, 60, 82, or 90. (B) Comparison of IL-2 promoter DNA binding ability to wild-type and modified NF- κ Bs. Controls included wild-type NF- κ B in the absence and presence of GTP (lanes 1 and 2), and NF κ Bs containing modified p50 subunits with pTyr at positions 44, 60, 82, or 90 (lanes 3, 4, 5, and 6, respectively). The statistical significance was determined using the Student’s t -test: wild-type vs pTyr44-p50, p > 0.05; wild-type vs pTyr60-p50, p < 0.001; wild-type vs pTyr82-p50, p < 0.001; and wild-type vs pTyr90-p50, p < 0.001.

Article Snippet: The relative intensities were determined using ImageQuant version 5.2 software (Molecular Dynamics) on the basis of the 32 P signal.

Techniques: Binding Assay, Modification, Software, Comparison

IL-2 promoter DNA binding of modified p50s expressed in a cell-free E. coli protein biosynthesizing system following treatment with a cytoplasmic lysate from activated Jurkat cells in the presence of GTP. Sample analysis was achieved by 5% native polyacrylamide gel electrophoresis (PAGE, 100 V for 1 h). Relative intensities were determined using ImageQuant software (version 5.2, Molecular Dynamics) on the basis of the 32 P signal. The band for wild-type NF- κ B in the presence of GTP was defined as 100%. Lane 1, wild-type p50; lanes 2–5, altered p50 subunits with pTyr at respective positions 44, 60, 82, or 90. Statistical significance was calculated with the Student’s t -test: p > 0.05 for all proteins.

Journal: ACS chemical biology

Article Title: Site-Selective Tyrosine Phosphorylation in the Activation of the p50 Subunit of NF- κ B for DNA Binding and Transcription

doi: 10.1021/acschembio.2c00678

Figure Lengend Snippet: IL-2 promoter DNA binding of modified p50s expressed in a cell-free E. coli protein biosynthesizing system following treatment with a cytoplasmic lysate from activated Jurkat cells in the presence of GTP. Sample analysis was achieved by 5% native polyacrylamide gel electrophoresis (PAGE, 100 V for 1 h). Relative intensities were determined using ImageQuant software (version 5.2, Molecular Dynamics) on the basis of the 32 P signal. The band for wild-type NF- κ B in the presence of GTP was defined as 100%. Lane 1, wild-type p50; lanes 2–5, altered p50 subunits with pTyr at respective positions 44, 60, 82, or 90. Statistical significance was calculated with the Student’s t -test: p > 0.05 for all proteins.

Article Snippet: The relative intensities were determined using ImageQuant version 5.2 software (Molecular Dynamics) on the basis of the 32 P signal.

Techniques: Binding Assay, Modification, Polyacrylamide Gel Electrophoresis, Software

Phosphorylation of wild-type p50 (A) and modified p50 (containing pTyr60) (B) in the presence of Jurkat cell lysate. The results were analyzed by 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The relative intensities were determined using ImageQuant software (version 5.2, Molecular Dynamics) based on the 32 P signal. 0 min: no Jurkat cell lysate was added; 0.5–60 min after Jurkat cell lysate addition. (C) Time-dependent phosphorylation of the wild-type and modified p50s in the presence of cell lysate from activated Jurkat cells. The band at 30 or 60 min was defined as 100%.

Journal: ACS chemical biology

Article Title: Site-Selective Tyrosine Phosphorylation in the Activation of the p50 Subunit of NF- κ B for DNA Binding and Transcription

doi: 10.1021/acschembio.2c00678

Figure Lengend Snippet: Phosphorylation of wild-type p50 (A) and modified p50 (containing pTyr60) (B) in the presence of Jurkat cell lysate. The results were analyzed by 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The relative intensities were determined using ImageQuant software (version 5.2, Molecular Dynamics) based on the 32 P signal. 0 min: no Jurkat cell lysate was added; 0.5–60 min after Jurkat cell lysate addition. (C) Time-dependent phosphorylation of the wild-type and modified p50s in the presence of cell lysate from activated Jurkat cells. The band at 30 or 60 min was defined as 100%.

Article Snippet: The relative intensities were determined using ImageQuant version 5.2 software (Molecular Dynamics) on the basis of the 32 P signal.

Techniques: Phospho-proteomics, Modification, Polyacrylamide Gel Electrophoresis, SDS Page, Software